Population structure and phylogenetic relationships in Brassica rapa L. subspecies by using isozyme markers

Abstract The present study aimed to assess population structure and phylogenetic relationships of nine subspecies Brassica rapa L. represented with thirty-five accessions cover a wide range species distribution area using isozyme analysis in order select more diverse as supplementary resources that can be utilized for improvement B. napus. Enzyme resulted detecting 14 putative polymorphic loci 27 alleles. Mean allele frequency 0.04 (rare alleles) was observed Cat4A Cat4B sub Oleifera accession CR 2204/79 trilocularis 2215/88 2244/88. highest genetic diversity measures were dichotoma, 1585/96 (the average (H0) expected heterozygosity (He), number alleles per locus (Ae)). These observations make this valuable resource included breeding programs the oilseed fixation index (F) is significantly higher than zero indicating significant deficiency heteozygosity. divergence among indicated very great differentiation (FST = 0.8972) which means about 90% distributed subspecies, while 10% within subspecies. This coincides low value gene flow (Nm 0.0287). ssp. oleifera (turnip rape) (sarson) grouped under one cluster morphological classification.